Institute Homepage DE Sign In

Back

Empirical Inference Article 2010

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution

Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES–ES and r-protein–ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

Author(s): Armache, J-P. and Jarasch, A. and Anger, AM. and Villa, E. and Becker, T. and Bhushan, S. and Jossinet, F. and Habeck, M. and Dindar, G. and Franckenberg, S. and Marquez, V. and Mielke, T. and Thomm, M. and Berninghausen, O. and Beatrix, B. and Söding, J. and Westhof, E. and Wilson, DN. and Beckmann, R.
Journal: Proceedings of the National Academy of Sciences of the United States of America
Volume: 107
Number (issue): 46
Pages: 19748-19753
Year: 2010
Month: November
Day: 0
Bibtex Type: Article (article)
DOI: 10.1073/pnas.1009999107
Digital: 0
Electronic Archiving: grant_archive
Links:

BibTex 

@article{ArmacheJAVBBJHDFMMTBBSWWB2011,
  title = {Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution},
  journal = {Proceedings of the National Academy of Sciences of the United States of America},
  abstract = {Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES–ES and r-protein–ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.},
  volume = {107},
  number = {46},
  pages = {19748-19753},
  month = nov,
  year = {2010},
  slug = {armachejavbbjhdfmmtbbswwb2011},
  author = {Armache, J-P. and Jarasch, A. and Anger, AM. and Villa, E. and Becker, T. and Bhushan, S. and Jossinet, F. and Habeck, M. and Dindar, G. and Franckenberg, S. and Marquez, V. and Mielke, T. and Thomm, M. and Berninghausen, O. and Beatrix, B. and S{\"o}ding, J. and Westhof, E. and Wilson, DN. and Beckmann, R.},
  month_numeric = {11}
}