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Optimized dualCEST-MRI for imaging of endogenous bulk mobile proteins in the human brain
{Recently we demonstrated that a selective detection of endogenous bulk mobile proteins in living tissue can be realized by the novel approach of dual-frequency irradiation CEST (dualCEST)-MRI1 without contamination of saturation transfer effects of metabolites, lipids and semi-solids. For this approach, specificity is achieved by measuring the intramolecular magnetization transfer (i.e. saturation crosstalk T) between CEST signals resonating at two different frequency offsets $\Delta$$\omega$ and $\Delta$$\omega$c (Fig. 1a). Such a non-invasive imaging technique may be of particular interest for the detection of pathological alterations of protein expression, such as in neurodegenerative diseases or cancer. Until now, application in clinical trials was prevented by the inherently small signal-to-noise ratio (SNR) in comparison to conventional CEST approaches. Here, we present further developments in signal preparation, image acquisition and post-processing techniques enabling dualCEST examinations in a reasonable and clinicallyrelevant time frame.}
@misc{item_3038355, title = {{Optimized dualCEST-MRI for imaging of endogenous bulk mobile proteins in the human brain}}, booktitle = {{7th International Workshop on Chemical Exchange Saturation Transfer (CEST 2018)}}, abstract = {{Recently we demonstrated that a selective detection of endogenous bulk mobile proteins in living tissue can be realized by the novel approach of dual-frequency irradiation CEST (dualCEST)-MRI1 without contamination of saturation transfer effects of metabolites, lipids and semi-solids. For this approach, specificity is achieved by measuring the intramolecular magnetization transfer (i.e. saturation crosstalk T) between CEST signals resonating at two different frequency offsets $\Delta$$\omega$ and $\Delta$$\omega$c (Fig. 1a). Such a non-invasive imaging technique may be of particular interest for the detection of pathological alterations of protein expression, such as in neurodegenerative diseases or cancer. Until now, application in clinical trials was prevented by the inherently small signal-to-noise ratio (SNR) in comparison to conventional CEST approaches. Here, we present further developments in signal preparation, image acquisition and post-processing techniques enabling dualCEST examinations in a reasonable and clinicallyrelevant time frame.}}, pages = {12}, year = {2018}, slug = {item_3038355}, author = {Breitling, J and Goerke, S and Zaiss, M and Soehngen, Y and Deshmane, A and Herz, K and Boyd, P and Ladd, ME and Bachert, P} }